Resistance to antibiotics and presence of plasmid in enterobacteria and staphylococcus aureus in the sector of diet of a public hospital

DOI: 10.15343/0104-7809.20153902147156

Abstract

The occurrence of antibiotic-resistant bacteria has become more frequent and the presence of these microorganisms in
hospitals exposes patients to serious risks. The presence of enterobacteria resistant to antibiotics and the presence of plasmid
DNA in the isolates were evaluated in a sector of dietary action a Brazilian public hospital. The enumeration and isolation
of enterobacteria were done in samples collected from food handlers, utensils, and food processing surfaces. Biochemical
identification of the isolates was done, and serum confirmations as well when necessary. The susceptibility to antibiotics of
therapeutic use was determined by the diffusion technique in agar and plasmidial DNA detection was done by agarose gel
electrophoresis. Fourteen genera and 27 species of enterobacteria and others Gram negative bacteria were identified out of
150 isolates obtained. Seventy-four isolates (58%) presented resistance to antibiotics. Seventy-four percent of the antibiotic
resistant isolates had palsmidial DNA. There was a significant difference between isolates resistant to ampicillin (p=0.00)
and kanamycin (p=0.03) in relation to sources of the samples. The presence of plasmidial DNA was more prevalence in
isolates resistant to antibiotics, but it was found that this correlation was significant only for the antibiotic tetracycline.
These results show how important aspects of the risk of propagation of antibiotic resistant bacteria, the majority of plasmid
DNA carrier, for processed foods in this environment and may result in hospital infections and contribute to the failure of
antimicrobial therapies.